Rabbit Polyclonal Antibody
Catalog No. A16-63BR
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Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).
1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
Recognizes the AKT1, AKT2, and AKT3 protein
Western blot AKT1, AKT2, and AKT3 from human and
AKT1, AKT2, and AKT3 from other species may also be detectable.
Synthetic peptide corresponding to amino acids 313-317 of human AKT1 (protein accession number P31749)
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot with Anti-AKT (pan) (1:1000) using 15 ng of human recombinant AKT1, AKT2, and AKT3.
Representative western blot with Anti-AKT (pan) (1:1000) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.
Zenga in et al., Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt Molecular Biology of the Cell September 2014 10.1091/mbc.E14-01-0004
Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009
Cancer, Cell Cycle, Inflammation, Neurobiology