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Anti-AKT (pan)

Rabbit Polyclonal Antibody
Catalog No. A16-63BR


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Catalog No. Pack Size Price (USD)
A16-63BR-100 100 ug $270
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Overview:

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).

References:

1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.


Specificity:

Recognizes the AKT1, AKT2, and AKT3 protein


Cross Reactivity:

Western blot AKT1, AKT2, and AKT3 from human and mouse cells

AKT1, AKT2, and AKT3 from other species may also be detectable.


Host :

Rabbit


Immunogen:

Synthetic peptide corresponding to amino acids 313-317 of human AKT1 (protein accession number P31749)


Purification:

Affinity chromatography


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot with Anti-AKT (pan) (1:1000) using 15 ng of human recombinant AKT1, AKT2, and AKT3.


Sample Data:

Representative western blot with Anti-AKT (pan) (1:1000) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.



Product Datasheets



RESEARCH AREAS

Cancer, Cell Cycle, Inflammation, Neurobiology


RELATED PRODUCTS


  AKT1, Active, A16-10G

  AKT1, Unactive, A16-14G

  Anti-AKT3, A18-63R

  AKT2, Active, A17-10G


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