MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal (1). Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division (2).
MYLK, KRP, MLCK108, MLCK210, MSTP083, FLJ12216, DKFZp686I10125
1. Gao, Y. et al: Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction. IUBMB Life. 2001 Jun;51(6):337-44.
2. Matsumura, F. et al: Role of myosin light chain phosphorylation in the regulation of cytokinesis. Cell Struct Funct. 2001 Dec;26(6):639-44.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Schneidewind T et al., The Pseudo Natural Product Myokinasib Is a Myosin Light Chain Kinase 1 Inhibitor with Unprecedented Chemotype. Cell Chemical Biology April 2019 10.1016/j.chembiol.2018.11.014
J. J. Cadete Virgilio et al., Ischemia/reperfusion-induced myosin light chain 1 phosphorylation increases its degradation by matrix metalloproteinase 2 FEBS Journal June 2012 10.1111/j.1742-4658.2012.08622.x
S Saha et al., N-terminal polar amino acids of the C2 insert of nonmuscle myosin II-C2 regulate its functional properties. FEBS Letters December 2016 10.1002/1873-3468.12446
Nyeo Chuna Jung et al., Schisandrin B suppresses TGFβ1-induced stress fiber formation by inhibiting myosin light chain phosphorylation Journal of Ethnopharmacology March 2014 10.1016/j.jep.2014.01.024
Cancer, Invasion/Metastasis, Ser/Thr Kinases