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AMPK (A2/B2/G1), Active

Recombinant full-length human AMPK (combination of A2/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.
Catalog No. P49-10GH


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Catalog No. Pack Size Price (USD)
P49-10GH-05 5 ug $435
P49-10GH-10 10 ug $655
P49-10GH-BULK BULK Contact Us  


Overview:

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.


Gene Aliases:

Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G1: PRKAG1, AMPKG, MGC8666


Genbank Number:


References:


1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.

2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~92kDa (A2), ~62kDa (B2), and ~68Da (G1)



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases


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