Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. T29-34G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| T29-34G-20 | 20 ug | $215 | |
| T29-34G-50 | 50 ug | $435 | |
| T29-34G-BULK | BULK | Contact Us |
Overview:
TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. TP53RK belongs to a small subfamily of atypical protein kinases. TP53RK is expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes and lower expression of TP53RK is found in heart, kidney, and spleen (1). PRPK interacts with CGI-121 and co-precipitation of p53 with PRPK is inhibited by adding recombinant CGI-121 in vitro. CGI-121 may act as an inhibitor of the PRPK-p53 interaction (2). TP53RK is activated by another kinase, Akt/PKB, which phosphorylates TP53RK at Ser250.
Gene Aliases:
BUD32; C20orf64; dJ101A2; Nori-2; Nori-2p
Genbank Number:
References:
1. Abe, Y.et.al: Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes. J. Biol. Chem. 276: 44003-44011, 2001.
2. Miyoshi, A.et.al: Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. Biochem. Biophys. Res. Commun. 303: 399-405, 2003.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~55 kDa
Ling Chau Jenny Fung et al., A crucial role for bone morphogenetic protein-Smad1 signalling in the DNA damage response Nature Communications May 2012 10.1038/ncomms1832
A Serrano M et al., DNA-PK, ATM and ATR collaboratively regulate p53-RPA interaction to facilitate homologous recombination DNA repair Oncogene May 2013 10.1038/onc.2012.257
S Mondal et al., A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Analytical Biochemistry October 2016 10.1016/j.ab.2016.06.016
Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, p38 Pathway
STAY CONNECTED
Fax: 1-604-232-4601
Toll Free: 1-866-954-6273
info@signalchem.com