LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis which is phosphorylated in a cell-cycle dependent manner. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity which indicating a role as a negative regulator of CDC2/cyclin A and the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding (1). LATS1 act as a tumor suppressor and play an important role in the development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments (2).
1. Tao, W.et.al: Human homologue of the Drosophila melanogaster lats tumour suppressor modulates CDC2 activity. Nature Genet. 21: 177-181, 1999.
2. St. John. et.al: Mice deficient of Lats1 develop soft-tissue sarcomas, ovarian tumours and pituitary dysfunction. Nature Genet. 21: 182-186, 1999.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
P. Lucas Eliana et al., The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells Journal of Cell Biology June 2013 10.1083/jcb.201210073
Yang X et al., A kinome-wide screen using a NanoLuc LATS luminescent biosensor identifies ALK as a novel regulator of the Hippo pathway in tumorigenesis and immune evasion Faseb Journal August 2019 10.1096/fj.201901343R
Keder Alyona et al., The Hippo Pathway Core Cassette Regulates Asymmetric Cell Division Current Biology November 2015 10.1016/j.cub.2015.08.064
B. Dewey Evan et al., Warts Phosphorylates Mud to Promote Pins-Mediated Mitotic Spindle Orientation in Drosophila, Independent of Yorkie Current Biology November 2015 10.1016/j.cub.2015.09.025
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