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PKC alpha, Active

Recombinant full-length xenopus PKCalpha was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. P61-10G


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Catalog No. Pack Size Price (USD)
P61-10G-05 5 ug $160
P61-10G-10 10 ug $226
P61-10G-BULK BULK Contact Us  


Overview:

PKCα is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control(1). PKCα has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes (2).


Gene Aliases:

PRKCA; MGC82897


Genbank Number:


References:


1. Coussens, L. et al: Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986.

2. Braz, J C. et al: PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nature Med. 10: 248-254, 2004.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~103 kDa



 Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028

 Dilworth David et al., The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stability Nucleir Acid Research 10.1093/nar/gky008

 A. Karim Zubair et al., IKB kinase phosphorylation of SNAP-23 controls platelet secretion Blood May 2013 10.1182/blood-2012-11-470468

 X Gan et al., Evidence for direct activation of mTORC2 kinase activity by phosphatidylinositol 3,4,5-trisphosphate Journal of Biological Chemistry April 2011 10.1074/jbc.M110.195016

 Stratford AL et al., Y-box binding protein-1 serine 102 is a downstream target of p90 ribosomal S6 kinase in basal-like breast cancer cells Breast Cancer June 2008 10.1186/bcr2202

 H Yamada et al., Possible role of cortactin phosphorylation by protein kinase C? in actin?bundle formation at growth cone Biology of the Cell September 2015 10.1111/boc.201500032

 M Tandon et al., SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest. PLoS One March 2015 10.1371/journal.pone.0119346

 Tandon Manuj et al., New Pyrazolopyrimidine Inhibitors of Protein Kinase D as Potent Anticancer Agents for Prostate Cancer Cells PLoS One September 2013 10.1371/journal.pone.0075601

 Tandon Manuj et al., A Targeted Library Screen Reveals a New Inhibitor Scaffold for Protein Kinase D PLoS One September 2012 10.1371/journal.pone.0044653

 J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245

 Qiu Chen Jin et al., Absolute quantitation of endogenous proteins with precision and accuracy using a capillary Western system Analytical Biochemistry November 2013 10.1016/j.ab.2013.07.022

 Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112

 Jinchao Zhang et al., Functional roles for post-translational modifications of t-SNAREs in platelets Thesis University of Kentucky April 2016 10.13023/ETD.2016.044


RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases


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