SRPK2 is a member of the serine/arginine (SR) protein-specific kinase family that are cell cycle-regulated protein kinases which phosphorylate SR domain-containing proteins in nuclear speckles and mediate the pre-mRNA splicing events (1). SRPK2 knock down results in hypophosphorylation of the serine/arginine domain-containing human PRP28 protein thereby destabilizing PRP28 association with the tri-snRNP. RNAi-mediated depletion in HeLa cells showed that SRPK2 is essential for cell viability, and it is required for spliceosomal B complex formation (2).
1. Wang, H. Y. Et al; SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J. Cell Biol. 140: 737-750, 1998.
2. Mathew, R. et al: Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome.Nat Struct Mol Biol. 2008 May;15(5):435-43.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Cancer, Inflammation, Neurobiology, Ser/Thr Kinases