Recombinant full-length human PKAcalpha was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. P51-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P51-10G-05 | 5 ug | $226 | |
| P51-10G-10 | 10 ug | $325 | |
| P51-10G-BULK | BULK | Contact Us |
Overview:
The catalytic subunit C-alpha of PKA (PKAcα) is a member of the Ser/Thr protein kinase family and has been assigned to chromosome region 19p13.1 (1). Null mutation in PKAcα leads to early postnatal lethality in the majority of C-alpha knockout mice. Surprisingly, a small percentage of C-alpha knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-beta levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity (2).
Gene Aliases:
PKAa; cAPKa; MGC48865; MGC102831
Genbank Number:
References:
1. Tasken, K. et al: The gene encoding the catalytic subunit C-alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1. Genomics 36: 535-538, 1996.
2. Skalhegg, BS. Et al: Mutation of the C-alpha subunit of PKA leads to growth retardation and sperm dysfunction. Molec. Endocr. 16: 630-639, 2002.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
|
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
|
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~69 kDa
Fukuda Koichi et al., The Pseudoactive Site of ILK Is Essential for Its Binding to ?-Parvin and Localization to Focal Adhesions Molecular Cell December 2009 10.1016/j.molcel.2009.11.028
A Thorne Curtis et al., Small-molecule inhibition of Wnt signaling through activation of casein kinase 1α Nature Chemical Biology November 2010 10.1038/nchembio.453
GiovannaDi Nardo,AndreaBandino,InesBarone RobertaBaravalle et al., Impact of R264C and R264H polymorphisms in human aromatase function J Steroid Biochem Mol Biol.? October 2001 10.1016/j.jsbmb.2016.09.022
Yamaguchi Fuminori et al., S100 Proteins Modulate Protein Phosphatase 5 FunctionA LINK BETWEEN CA2+ SIGNAL TRANSDUCTION AND PROTEIN DEPHOSPHORYLATION Journal of Biological Chemistry April 2012 10.1074/jbc.M111.329771
S Shah et al., Regulation of FcRγ function by site-specific serine phosphorylation. Journal of Leukocyte Biology September 2016 10.1189/jlb.2AB0516-228R
R Baravalle et al., Impact of R264C and R264H polymorphisms in human aromatase function. Journal of Steroid Biochemistry and Molecular Biology October 2016 10.1016/j.jsbmb.2016.09.022
Martic? Sanela et al., Electrochemical investigations into Tau protein phosphorylations Analyst March 2012 10.1039/c2an35097a
J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., PLPP/CIN-mediated NF2-serine 10 dephosphorylation regulates F-actin stability and Mdm2 degradation in an activity-dependent manner Cell Death & Disease January 2021
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases
PKAc gamma, Active, P53-10G
CREB1 Protein, C49-55G
AMPK (A1/B2/G1), Active, P50-10H
PRKAR1A Protein, P57-30H
STAY CONNECTED
Fax: 1-604-232-4601
Toll Free: 1-866-954-6273
info@signalchem.com