Recombinant full-length human p110 delta (E1021K) and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Catalog No. P30-122H
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PI3K (p110 delta /p85 alpha) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110delta/p85alpha) plays a role in oncogenic transformation and is consistently expressed at a high level in blast cells from AML. PI3K (p110delta/p85alpha) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110delta/p85alpha) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
p110 alpha: p110 delta, p85 alpha: PIK3R1, GRB1, p85-ALPHA
1. Vanhaesebroeck, B. et.al: p110-delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Nat. Acad. Sci. 94: 4330-4335, 1997.
2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
P110 delta: 111 kDa and p85 alpha: 86 kDa
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases