USP28 (ubiquitin carboxyl-terminal hydrolase 28) is a deubiquitinase belonging to the family of deubiquitinating enzymes (DUBs) and plays a role in the DNA damage pathway by specifically deubiquitinating proteins of the pathway such as CLSPN. USP2 deubiquitinates ZNF304, inhibiting its degradation and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in various colorectal cancers (CRC) cells (1). USP28 counteracts the activity of E3 ligases, antagonizes FBW7 and a component of the SCF-type E3 ubiquitin ligase that targets key transcriptional factors to ubiquitin-directed proteasome degradation, thus stabilizing substrates such as oncogenic c-MYC (2). This may lead to enhanced proliferation, carcinogenesis, and metastasis.
1. Serra, Ryan W et al: A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. eLife 3: e02313, 2014.
2. Wang, X. et al: Targeting deubiquitinase USP28 for cancer therapy. Cell Death & Disease 9: 186, 2018.
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder