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USP28, Active

Recombinant human USP28 (155-675a.a.) was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. U528-381G


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Catalog No. Pack Size Price (USD)
U528-381G-05 5 ug $226
U528-381G-10 10 ug $325
U528-381G-BULK BULK Contact Us  


Overview:

USP28 (ubiquitin carboxyl-terminal hydrolase 28) is a deubiquitinase belonging to the family of deubiquitinating enzymes (DUBs) and plays a role in the DNA damage pathway by specifically deubiquitinating proteins of the pathway such as CLSPN. USP2 deubiquitinates ZNF304, inhibiting its degradation and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in various colorectal cancers (CRC) cells (1). USP28 counteracts the activity of E3 ligases, antagonizes FBW7 and a component of the SCF-type E3 ubiquitin ligase that targets key transcriptional factors to ubiquitin-directed proteasome degradation, thus stabilizing substrates such as oncogenic c-MYC (2). This may lead to enhanced proliferation, carcinogenesis, and metastasis.


Gene Aliases:

KIAA1515


Genbank Number:


References:


1. Serra, Ryan W et al: A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. eLife 3: e02313, 2014.

2. Wang, X. et al: Targeting deubiquitinase USP28 for cancer therapy. Cell Death & Disease 9: 186, 2018.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

85 kDa



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder


RELATED PRODUCTS


  DUB Reaction Buffer (4X), D01-09

  DUB Substrate I, U06-57


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