Rabbit Polyclonal Antibody
Catalog No. S56-365R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S56-365R-100 | 100 ug | $325 | |
S56-365R-BULK | BULK | Contact Us |
Overview:
Signal transducers and activators of transcription (STAT)5A is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). STAT proteins are responsible for the differentiation of helper T cells, thus playing key roles in regulating immune functions. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and results in increased resistance to apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2). STAT5A is phosphorylated at Ser780 in response to IL-2 stimulation (3).
References:
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.
3. Xue H H. et al: Serine phosphorylation of Stat5 proteins in lymphocytes stimulated with IL-2. Int Immunol. 2002 Nov;14(11):1263-71.
Specificity:
Recognizes the MEK1/2 protein phosphorylated at serine 780
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser780
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of extracts from HeLa cells using anti-phospho-STAT5A (Ser780) antibody.
Sample Data:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-STAT5A (Ser780) antibody.
A. Cumaraswamy Abbarna et al., Nanomolar-Potency Small Molecule Inhibitor of STAT5 Protein ACS Medical Chemistry Letters September 2014 10.1021/ml500165r
Zeidan N et al., Magnetic bead-based electrochemical detection of interaction between epigallocatechin-3-gallate and STAT proteins Analytical Methods March 2015 10.1039/c5ay00169b
Zhanga Xiaolei et al., Orally bioavailable small-molecule inhibitor of transcription factor Stat3 regresses human breast and lung cancer xenografts PNAS January 2012 10.1073/pnas.1121606109
Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway
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