Specificity:

Recognizes the PLCG1 protein phosphorylated at tyrosine 783

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr783

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 10min) using PLCG1 antibody (lanes 1 and 2) and anti-phospho-PLCG1 (Tyr783) antibody (lanes 3 and 4).

References :

1. Ye, K. et al: Phospholipase C-gamma-1 is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE. Nature 415: 541-544, 2002.

2. Kim H K. et al: PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell. 1991 May 3;65(3):435-41.

3. Wang Z. et al: Requirement for phospholipase c-gamma1 enzymatic activity in growth factor-induced mitogenesis. Mol Cell Biol. 1998 Jan;18(1):590-7.