Specificity:

Recognizes the KDR protein phosphorylated at tyrosine 1214

Cross Reactivity:

Human and Mouse

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1214

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using phosphor-KDR (Tyr1214) antibody.

References :

1. Neufeld, G. et al: Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 1999 Jan;13(1):9-22.

2. Zhu, Z. et al: Inhibition of tumor growth and metastasis by targeting tumor-associated angiogenesis with antagonists to the receptors of vascular endothelial growth factor. Invest New Drugs. 1999;17(3):195-212.

3. Lamalice L. et al: Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF. J Biol Chem. 2006 Nov 10;281(45):34009-20.