Anti-phospho-HER2 (Tyr877)
Rabbit Polyclonal Antibody
Catalog No. E27-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| E27-65R-100 | 100 ug | $270 | |
| E27-65R-BULK | BULK | Contact Us |
Rabbit Polyclonal Antibody
Catalog No. E27-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| E27-65R-100 | 100 ug | $270 | |
| E27-65R-BULK | BULK | Contact Us |
Overview:
HER2 is a glycoprotein tyrosine kinase receptor that has extensive homology to the epidermal growth factor receptor. Overexpression of HER2 in NIH 3T3 cells results in cellular transformation and tumorigenesis (1). HER2 is amplified in about 30% of primary human breast malignancies and is associated with the most aggressive tumors. These tumors characteristically display uncontrolled proliferation, resistance to apoptosis and increased motility (2). HER2 activity is regulated in part by phosphorylation at Tyr877.
References:
1. Hudziak, R M. et al: Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7159-63.
2. Badache, A. et al: The ErbB2 Signaling Network as a Target for Breast Cancer Therapy. J Mammary Gland Biol Neoplasia. 2006 Jan;11(1):13-25.
Specificity:
Recognizes the HER2 protein phosphorylated at tyrosine 877
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr877
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of extracts from MDA-MB-231 cells untreated or treated with EGF (200ng/ml, 5min), using HER2 antibody (lanes 1 and 2) and anti-phospho-HER2 (Tyr877) antibody (lanes 3 and 4).
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Sample Data:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-HER2 (Tyr877) antibody.
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Imami Koshi et al., Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways Molecular and Cellular Proteomics September 2012 10.1074/mcp.M112.019919
Y Dunkel et al., Prognostic impact of total and tyrosine phosphorylated GIV/Girdin in breast cancers FASEB Journal November 2016 10.1096/fj.201600500
L. Disis Mary et al., HER-2/neu vaccine-primed autologous T-cell infusions for the treatment of advanced stage HER-2/neu expressing cancers Cancer Immunology, Immunotherapy February 2014 10.1007/s00262-013-1489-4
A Chamas et al., Purification and immunodetection of the complete recombinant HER-2[neu] receptor produced in yeast. Protein Expression and Purification January 2015 10.1016/j.pep.2014.10.004
AKT/PKB Pathway, Cancer, ERK/MAPK Pathway, Receptor Tyrosine Kinases
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