Anti-phospho-ERK1/2 (Tyr204)
Rabbit Polyclonal Antibody
Catalog No. M29-65BR
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| M29-65BR-100 | 100 ug | $270 | |
| M29-65BR-BULK | BULK | Contact Us |
Rabbit Polyclonal Antibody
Catalog No. M29-65BR
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| M29-65BR-100 | 100 ug | $270 | |
| M29-65BR-BULK | BULK | Contact Us |
Overview:
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is a serine threonine kinase. It plays an integral role of cellular signaling during mitogenesis and differentiation of mitotic cells. ERK is presumed to have a key role in learning and memory (1,2,3). The activity of this kinase is regulated by phosphorylation at Thr202 and Tyr204 (4). Activated ERK1/2 translocates into the nucleus where it phosphorylates various transcription factors (e.g Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta).
References:
1. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
2. Johnson, G L. et al: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 202 298:1911-1912.
3. Tanoue, T J. et al: Molecular recognitions in the MAP kinase cascades. Cellular Signaling 2003 15:455-462.
4. Ahn, N G.: The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 1993 127-128:201-209.
Specificity:
Recognizes the ERK1/2 protein phosphorylated at tyrosine 204
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr204
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blots from NIH-3T3 cells using ERK1/2 antibody (lanes 1 and 2) and anti-phospho-ERK1/2 (Tyr204) antibody (lanes 3 and 4).
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Sample Data:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ERK1/2 (Tyr204) antibody.
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Li Saiqun et al., RAS/ERK Signaling Controls Proneural Genetic Programs in Cortical Development and Gliomagenesis Journal of Neuroscience February 2014 10.1523/JNEUROSCI.4077-13.2014
Tonga Junfeng et al., Phosphorylation of Rac1 T108 by Extracellular Signal-Regulated Kinase in Response to Epidermal Growth Factor: a Novel Mechanism To Regulate Rac1 Function Molecular and Cellular Biology November 2013 10.1128/MCB.00822-13
E. Franklin Norah et al., Differential phosphorylation of the phosphoinositide 3-phosphatase MTMR2 regulates its association with early endosomal subtypes Journal of Cell Science February 2013 10.1242/jcs.113928
Tyler Moore et al., IRF3 and ERK MAP-kinases control nitric oxide production from macrophages in response to poly-I:C FEBS Letters September 2013 10.1016/j.febslet.2013.07.025
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
Neise Denise et al., Evidence for a differential modulation of p53-phosphorylating kinases by the cyclin-dependent kinase inhibitor p21WAFI/C1P1 Cell Cycle September 2010 10.4161/cc.9.17.12799
AL Lasek et al., The Functional Significance of Posttranslational Modifications on Polo-Like Kinase 1 Revealed by Chemical Genetic Complementation. PLoS One February 2016 10.1371/journal.pone.0150225
Tong Junfeng et al., Phosphorylation and Activation of RhoA by ERK in Response to Epidermal Growth Factor Stimulation PLoS One January 2016 10.1371/journal.pone.0147103
N Sato et al., MEK and PI3K catalytic activity as predictor of the response to molecularity targeted agents in triple-negative breast cancer Biochemical and Biophysical Research Communications August 2017
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
R Pal et al., Inhibition of ERK1/2 Restores GSK3β Activity and Protein Synthesis Levels in a Model of Tuberous Sclerosis Sci Rep June 2017 10.1038/s41598-017-04528-5
R Pal et al., Inhibition of ERK1/2 Restores GSK3β Activity and Protein Synthesis Levels in a Model of Tuberous Sclerosis Sci Rep June 2017 10.1038/s41598-017-04528-5
K Fujita et al., HMGB1, a pathogenic molecule that induces neurite degeneration via TLR4-MARCKS, is a potential therapeutic target for Alzheimer's disease. Science Reports August 2016 10.1038/srep31895
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases
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