Rabbit Polyclonal Antibody
Catalog No. A10-565R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A10-565R-100 | 100 ug | $325 | |
A10-565R-BULK | BULK | Contact Us |
Overview:
The activating transcription factor ATF2 (also known CREBP1) is a member of the ATF/CREB family of leucine zipper transcription factors. These bind both AP-1 and CRE DNA response elements (1). ATF2 has two unique characteristics: it mediates adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (2). ATF2 is a target of the JNK signal transduction pathway (3). ATF2 is phosphorylated by JNK at two closely spaced threonine residues located in the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, ATF2 is a target for p38 MAPK phosphorylation.
References:
1. Maekawa, T. et al: Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J 1989 8:2023-2028.
2. Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element binding protein CRE-BP1. J Biol Chem. 1993 Feb 25;268(6):4259-66.
3. Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science, 1995 Jan 20;267(5196):389-93.
Specificity:
Recognizes the ATF2 protein phosphorylated at serine 112
Cross Reactivity:
Human and Mouse
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser112
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Immunohistochemistry image of paraffin-embedded human breast carcinoma tissue using the anti-phospho-ATF (Ser112) antibody.
Angiogenesis, Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Metabolic Disorder, Neurobiology, NfkB Pathway, p38 Pathway
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