Specificity:

Recognizes the PAK1/2/3 protein phosphorylated at threonine 402

Cross Reactivity:

Human, Mouse, Rat, Bovine and Canine

Host:

Rabbit, IgG

Immunogen:

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH

Purification:

Affinity Chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68kDa to ~70kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PAK1/2/3 (Thr402) antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-Ptase.