Specificity:

Recognizes the EPHB2 protein phosphorylated at tyrosine 473

Cross Reactivity:

Human, Mouse, Bovine, Chicken, Xenopus and Zebrafish

Host:

Rabbit, IgG

Immunogen:

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr473 conjugated to KLH

Purification:

Affinity Chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot of rat testes lysate showing specific immunolabeling of the ~46kDa EPHB2 phosphorylated at Tyr473 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-EPHB2 (Tyr473). The immunolabeling of the EPHB2 is completely eliminated by treatment with lambda-Ptase.