Anti-Phospho-RSK1 (Ser732)
Rabbit Polyclonal Antibody
Catalog No. R15-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| R15-65R-100 | 100 ug | $675 | |
| R15-65R-BULK | BULK | Contact Us |
Rabbit Polyclonal Antibody
Catalog No. R15-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| R15-65R-100 | 100 ug | $675 | |
| R15-65R-BULK | BULK | Contact Us |
Overview:
RSK1 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. RSK1 encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2).
References:
1. Moller, D E. et al: Human rsk isoforms: cloning and characterization of tissue-specific expression. Am. J. Physiol. 266: C351-C359, 1994.
2. Gross, S D. et al: Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90(Rsk). Science 286: 1365-1367, 1999.
Specificity:
Recognizes the human p90 RSK1 protein phopshorylated at Serine 732
Cross Reactivity:
Human, Mouse, Rat, Horse, Bovine and Dog
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to a region near the C-Terminal of the human RSK1 protein
Purification:
Immunoaffinity chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot using affinity purified Anti-Phospho-RSK1 (Ser732) (1:1000) shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1).
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MJ Lommel et al., L-plastin Ser5 phosphorylation breast cancer cells and in vitro is mediated by RSK downstream of the ERK/MAPK pathway. FASEB Journal March 2016 10.1096/fj.15-276311
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
M. Reipas Kristen et al., Luteolin is a novel p90 ribosomal S6 kinase (RSK) inhibitor that suppresses Notch4 signaling by blocking the activation of Y-box binding protein-1 (YB-1). Oncotarget February 2014 10.18632/oncotarget.834
A. Everley Robert et al., Increasing Throughput in Targeted Proteomics Assays: 54-Plex Quantitation in a Single Mass Spectrometry Run Analytical Chemistry May 2013 10.1021/ac400845e
A. Galana Jacob et al., Phosphoproteomic analysis identifies the tumor suppressor PDCD4 as a RSK substrate negatively regulated by 14-3-3 PNAS April 2014 10.1073/pnas.1405601111
Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases
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