Anti-Phospho-AURORA B (Thr 232)
Rabbit Polyclonal Antibody
Catalog No. A31-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| A31-65R-100 | 100 ug | $675 | |
| A31-65R-BULK | BULK | Contact Us |
Rabbit Polyclonal Antibody
Catalog No. A31-65R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| A31-65R-100 | 100 ug | $675 | |
| A31-65R-BULK | BULK | Contact Us |
Overview:
AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
References:
1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.
2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004.
Specificity:
Recognizes the human Aurora Kinas B protein phosphorylated at Threonine 232.
Cross Reactivity:
Human and Mouse
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to amino acids 223-234 of Human Aurora Kinase B protein.
Purification:
Affinity chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western Blot detection of Aurora B protein at 39 using Anti-Phospho-AURORA B (Thr2320 antibody (1:500). Lane 1 : Extract from COS7 cells treated with Nocodazole (1µg/ml, 16 hrs). Lane 2: Extract from COS7 cells treated with Nocodazole (1µg/ml, 16 hrs) and with the phosphopeptide immunogen.
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Sample Data:
Immunohistochemical analysis of formalin fixed, paraffin embedded human intestine (pH9) tissue (A) at 20x using 10 µg/mL Anti-phospho-AURORA B (Thr232). Analysis of formalin fixed, paraffin embedded human placenta pH9 tissue (A) at 20x using 10 µg/mL Anti-Phospho-AURORA B (Thr232)
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A Stützer et al., Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails. Molecular Cell January 2016 10.1016/j.molcel.2015.12.015
Mandala Ranadip et al., pERK 1/2 inhibit Caspase-8 induced apoptosis in cancer cells by phosphorylating it in a cell cycle specific manner Molecular Oncology March 2013 10.1016/j.molonc.2013.11.003
Kassardjian Ari et al., The Transcription Factor YY1 Is a Novel Substrate for Aurora B Kinase at G2/M Transition of the Cell Cycle PLoS One November 2012 10.1371/journal.pone.0050645
Z Xingyu et al., Quercetin suppresses lung cancer growth by targeting Aurora B kinase. Cancer Med. November 2016 10.1002/cam4.891
Cancer, Cell Cycle, Ser/Thr Kinases
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