Specificity:

Recognizes the APC1 protein phosphorylated at Serine 377

Cross Reactivity:

Human

Host Isotype / Clone#:

Rabbit, IgG

Immunogen:

The antibody was produced against synthesized peptide corresponding to amino acids 373-382 of human APC1 protein.

Purification:

Immunoaffinity chromatography followed by solid phase adsorption.

Stability:

1yr at –20^oC from date of shipment

Sample Data:

Western blot using Anti-Phospho-APC1 (Ser377) (1:1,000) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30 µg of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose.

Sample Data:

Anti-Phospho-APC1 (Ser377) antibody was used at 5.0 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides at 60X. Tissue was formalin-fixed and paraffin embedded.