Rabbit Polyclonal Antibody
Catalog No. J02-65R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
J02-65R-100 | 100 ug | $325 | |
J02-65R-BULK | BULK | Contact Us |
Overview:
JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway (1). JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon (2).
References:
1. Lacronique, V. Et al: A TEL-JAK2 fusion protein with constitutive kinase activity in human leukemia. Science, 1997; 278: 1309-1312.
2. Ho, J.M. et al: TEL-JAK2 constitutively activates the extracellular signal-regulated kinase (ERK), stress-activated protein/Jun kinase (SAPK/JNK), and p38 signaling pathways. Blood, 2002; 100: 1438-1448.
3. Feener EP, et al.(2004) Mol Cell Biol. 24(11): 4968-4978. Argetsinger LS, et al.(2004) Mol Cell Biol. 24(11): 4955-4967.
Specificity:
Recognizes the JAK2 protein phosphorylated at tyrosine 221
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 221 (Q-D-YP-H-I).
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-JAK2 (Tyr221)
Huang Yongqi et al., The Activity and Stability of the Intrinsically Disordered Cip/Kip Protein Family AreRegulated by Non-Receptor Tyrosine Kinases Journal of Molecular Biology January 2015 10.1016/j.jmb.2014.11.011
Zhang Jing et al., Dehydrocrenatidine is a Novel JAK Inhibitor Molecular Pharmacology January 2015 10.1124/mol.114.095208
Deng Yang et al., Global Analysis of Human Nonreceptor Tyrosine Kinase Specificity Using High-Density Peptide Microarrays Journal of Proteome Research August 2014 10.1021/pr500503q
JW Singer et al., Comprehensive kinase profile of pacritinib, a nonmyelosuppressive Janus kinase 2 inhibitor. Journal of Experimental Pharmacology August 2016 10.2147/JEP.S110702
Kesarwani Meenu et al., Targeting substrate-site in Jak2 kinase prevents emergence of genetic resistance Science Reports September 2015 10.1038/srep14538
Cancer, Cardiovascular Disease, Cytoplasmic Tyrosine Kinases, Inflammation, JAK/STAT Pathway
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