Specificity:

Recognizes the Histone H2A.X protein phosphorylated at serine 139

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X antibody (Lane 1+2) and Anti-phospho-Histone H2A.X (Ser139) (Lane 3+4)

Sample Data:

Immunofluorescence staining of methanol-fixing HeLa cells using Anti-phospho-Histone H2A.X (Ser139)

Sample Data:

Western blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-Histone H2A.X (Ser139)