SIRT3 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT3 being a member of class I. SIRT3 contains an N-terminal mitochondrial targeting signal and a central catalytic domain and localizes in the nuclei and mitochondria (1). SIRT3 has NAD(+)-dependent histone deacetylase activity with specificity for lys16 of H4 and, to a lesser extent, lys9 of H3. SIRT3 can repress transcription of a target gene in vivo when recruited to its promoter. Full-length SIRT3 is transported from the nucleus to the mitochondria during cell stress conditions (2).
SIR2L3, Sirtuin 3
1. Onyango, P. et al: SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria. Proc. Nat. Acad. Sci. 99: 13653-13658, 2002.
2. Scher, M. B. et al: SirT3 is a nuclear NAD(+)-dependent histone deacetylase that translocates to the mitochondria upon cellular stress. Genes Dev. 21: 920-928, 2007.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Cancer, Cell Cycle, Inflammation