SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterols synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation.
SIR2L, SIR2L2, Sirtuin 2
1. Luthi-Carter, R. Et al: SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7927-32.
2. North, B. J. et al: The human Sir2 ortholog, SIRT2, is an NAD(+)-dependent tubulin deacetylase. Molec. Cell 11: 437-444, 2003.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Choi Gildon et al., Discovery of a potent small molecule SIRT1/2 inhibitor with anticancer effects International Journal of Oncology July 2013 10.3892/ijo.2013.2035
J. Evans Nathan et al., Detection of Lysine Deacetylase Activities : Bioluminescent Assays Offer Distinct Advantages over Fluorescent Approaches Genetic Engineering & Biotechnology News October 2011 10.1089/gen.31.19.13
Cancer, Cell Cycle, Inflammation