Protein phosphatase methylesterase 1 (PPME1) conducts the demethylation of the catalytic subunit of protein phosphatase 2A (PP2A) and leads to inactivation of the phosphatase (1). It is demonstrated that PPME1 binds directly to the active site of PP2A and evicts the manganese ions required for the phosphatase activity (2). PPME1 is also reported to protect ERK activity in human malignant glioma (3).
1. Ogris, E., et al. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A. J Biol Chem. 274(20):14382-91, 1999.
2. Xing, Y., et al. Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell. 133(1):154-63, 2008.
3. Puustinen, P., et al. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma. Cancer Res. 69(7):2870-7, 2009.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
There are no related publications available for this product.