Recombinant human HGK (1-328) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M26-11G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M26-11G-05 | 5 ug | $226 | |
M26-11G-10 | 10 ug | $325 | |
M26-11G-BULK | BULK | Contact Us |
Overview:
HGK is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK (1). The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-α signaling. HGK-dependent signaling inhibits PPARγ-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport (2).
Gene Aliases:
MAP4K4, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687
Genbank Number:
References:
1. Yao, Z. et.al: A novel human STE20-related protein kinase, HGK, that specifically activates the c-jun N-terminal kinase signaling pathway. J. Biol. Chem. 274: 2118-2125, 1999.
2. Tang, X. et.al: An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPAR-gamma, adipogenesis, and insulin-responsive hexose transport. Proc. Nat. Acad. Sci. 103: 2087-2092, 2006.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~64 kDa
Zhenkun L et al., Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents Science Advances September 2020 10.1126/sciadv.abb2630
Sunil Kumar Singh et al., MAP4K4 promotes pancreatic tumorigenesis via phosphorylation and activation of mixed lineage kinase 3 Oncogene September 2021 10.1038/s41388-021-02007-w
Das S et al., Mixed lineage kinase 3 promotes breast tumorigenesis via phosphorylation and activation of p21-activated kinase 1. Oncogene January 2019 10.1038/s41388-019-0690-0
Lee Rubin et al., METHODS, COMPOSITIONS AND KITS FOR PROMOTING MOTOR NEURON SURVIVAL AND TREATING AND DIAGNOSING NEURODEGENERATIVE DISORDERS Patent US 20160082015 April 2016
Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, NfkB Pathway, Ser/Thr Kinases
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