Recombinant full-length human MYLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M64-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M64-10G-05 | 5 ug | $226 | |
M64-10G-10 | 10 ug | $325 | |
M64-10G-BULK | BULK | Contact Us |
Overview:
MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure (1). Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly (2). Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.
Gene Aliases:
caMLCK; MLCK, MLCK2, MGC126319, MGC126320
Genbank Number:
References:
1. Chan, J. Y. et al:Identification of cardiac-specific myosin light chain kinase. Circ. Res.2008; 102: 571-580.
2. Seguchi, O. et al: A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest. 2007;117: 2812-2824
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~135 kDa
S Saha et al., N-terminal polar amino acids of the C2 insert of nonmuscle myosin II-C2 regulate its functional properties. FEBS Letters December 2016 10.1002/1873-3468.12446
et al., CaMKII activity contributes to Homeometric Autoregulation of the heart: a novel mechanism for the Anrep effect The Journal of physiology November 2021
Cardiovascular Disease, PKA/PKC Pathway, Ser/Thr Kinases
STAY CONNECTED
Fax: 1-604-232-4601