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TEV (Tobacco Etch Virus) Protease, Active

Recombinant TEV (Tobacco Etch Virus) protease (2038-2279aa) was expressed in E. coli cells using an N-terminal His tag.
Catalog No. T525-31H


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Catalog No. Pack Size Price (USD)
T525-31H-02 0.2 ml $116
T525-31H-2 2 ml $490
T525-31H-20 20 ml $3185
T525-31H-BULK BULK Contact Us  


Overview:

Recombinant Tobacco Etch Virus (TEV) Protease is derived from the naturally occurring 28 kDa Nla recombinant protease. TEV Protease is commonly used for removing affinity tags from purified recombinant fusion proteins. TEV protease is highly site-specific, targeting the consensus sequence EXXYXQ(G/S). Cleavage occurs between Q and G/S. The most common TEV target sequences are ENLYFQG or ENLYFQS. The enzyme retains activity in solutions containing 1M Urea, 1-5mM DTT, beta-Mercaptoethanol, EDTA and PMSF, Aprotinin, Leupeptin and other protease inhibitors. The 6-His tag enables easy removal of the protease.


Gene Aliases:

NI-a, NIa-pro, 49kDa-Pro



Sample Data:


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Enzyme Commission Number:

EC 3.4.22.44


Storage, Stability, and Shipping:

Store product at –70oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.


Digestion Conditions:

Catalytic pH range: 5.5 ~ 8.5; catalytic temperature range: 4 ~ 30oC



Product Datasheets



Safety Datasheet



There are no related publications available for this product.


SIGNALCHEM BIOTECH INC.


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