Recombinant TEV (Tobacco Etch Virus) protease (2038-2279aa) was expressed in E. coli cells using an N-terminal His tag.
Catalog No. T525-31H
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Recombinant Tobacco Etch Virus (TEV) Protease is derived from the naturally occurring 28 kDa Nla recombinant protease. TEV Protease is commonly used for removing affinity tags from purified recombinant fusion proteins. TEV protease is highly site-specific, targeting the consensus sequence EXXYXQ(G/S). Cleavage occurs between Q and G/S. The most common TEV target sequences are ENLYFQG or ENLYFQS. The enzyme retains activity in solutions containing 1M Urea, 1-5mM DTT, beta-Mercaptoethanol, EDTA and PMSF, Aprotinin, Leupeptin and other protease inhibitors. The 6-His tag enables easy removal of the protease.
NI-a, NIa-pro, 49kDa-Pro
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number:
Storage, Stability, and Shipping:
Store product at –70oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Catalytic pH range: 5.5 ~ 8.5; catalytic temperature range: 4 ~ 30oC
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