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Lys-c (lysyl-endopeptidase), Active

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endo-peptidase) (206-473aa) was expressed in E. coli cells.
Catalog No. L585-31N


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Catalog No. Pack Size Price (USD)
L585-31N-05 0.5 mg $160
L585-31N-5 5 mg $930
L585-31N-BULK BULK Contact Us  


Overview:

Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 – 37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.


Gene Aliases:

API, Protease I, Lysyl endopeptidase



Sample Data:


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Enzyme Commission Number:

EC 3.4.21.50


Storage, Stability, and Shipping:

Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.


Digestion Conditions:

Catalytic pH range: 9.0 ~ 9.5; catalytic temperature range: 30 ~ 37oC



Product Datasheets



There are no related publications available for this product.


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