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Immobilized Lys-c, Agarose

Recombinant tag-free Lys-c (Lysyl-Endopeptidase) was expressed in E. coli cells.
Catalog No. L585-31AN


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Catalog No. Pack Size Price (USD)
L585-31AN-1 1 mg $457
L585-31AN-5 5 mg $1260
L585-31AN-BULK BULK Contact Us  


Overview:

Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 – 37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.

Immobilized Lysyl-Endopeptidase is covalently cross-linked to an agarose matrix circumventing the need for enzyme removal after cleavage. The resin can be reused 10-20 times after regenerating when proper storage conditions are followed.


Gene Aliases:

API, Protease I, Lysyl endopeptidase



Matrix:

cross-linked agarose


Average Particle Size:

90 μm


Ligand Density:

1 mg protein / ml matrix


Maximum Flow Rate, Pressure:

100 cm/h, 0.3 bar


Sample Data:


Enzyme Commission Number:

EC 3.4.21.50


Storage, Stability, and Shipping:

Store product at 2 - 4oC for up to 6 months.


Digestion Conditions:

Catalytic pH range: 8.0 ~ 9.5


Catalytic temperature range:

15 ~ 37oC


Enzyme:

Substrate ratio: 1:200 ~1:2,000



Product Datasheets



There are no related publications available for this product.


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