Carboxypeptidase B (CPB) is a metal-containing pancreatic endopeptidase that catalyzes the successive release of basic carboxy-terminal residues, such as Arg and Lys. CPB also targets other amino acids, albeit at a slower rate. These secondary target residues include valine, leucine, isoleucine, asparagine, glycine, and glutamine. CPB depends on Zn2+ to promote enzymatic activity. Conversely, the enzyme is inhibited by some heavy metals, including Cu2+, Hg2+; metal chelating agents such as EDTA and competing Arg and Lys residues. CPB functions between pH 5-12, with an optimal pH of 8. Enzyme activity also increases with rises in temperature. CPB is used in the production of recombinant human insulin and monoclonal antibody/IgG1 (mAb) processing.
CPB1; CPB2; Carboxylase B
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number:
Storage, Stability, and Shipping:
Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Catalytic pH range: 5.0~12.0; maximum temperature: 60oC
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