Recombinant human NAE1 (2-end) and full-length human UBA3 were expressed by baculovirus in Sf9 insect cells.
Catalog No. U203-380G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
U203-380G-05 | 5 ug | $259 | |
U203-380G-10 | 10 ug | $380 | |
U203-380G-BULK | BULK | Contact Us |
Overview:
NAE1/UBA3 is a heterodimeric E1 (Ub-activating)–like complex that activates NEDD8. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (3).
Gene Aliases:
NAE1 - APPBP1 UBA3 - UBE1C, UBA3, hUBA3
References:
1. Zhou, L. et al: Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 18: 77, 2019. 2. Gong, L. et al: Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 23 (274):12036-42, 1999. 3. Soucy, T.A. et al: The NEDD8 conjugation pathway and its relevance in cancer biology and therapy. Genes Cancer. 1: 708-716, 2010.
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity:
The specific activity of NAE1/UBA3 was determined to be 6.4 nmol/min/mg as per activity assay protocol.
Purity:
The purity of NAE1/UBA3 was determined to be >70% by densitometry. NAE1 approx. MW 65 kDa. UBA3 approx. MW 73 kDa.
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Cell Cycle
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