Overview:

NAE1/UBA3 is a heterodimeric E1 (Ub-activating)–like complex that activates NEDD8. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (3).

Gene Aliases:

NAE1 - APPBP1 UBA3 - UBE1C, UBA3, hUBA3

Genbank Number:

NM_003905
BC022853

References:

1. Zhou, L. et al: Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 18: 77, 2019. 2. Gong, L. et al: Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 23 (274):12036-42, 1999. 3. Soucy, T.A. et al: The NEDD8 conjugation pathway and its relevance in cancer biology and therapy. Genes Cancer. 1: 708-716, 2010.