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2019-nCoV NSP14 Methyltransferase, Active

Recombinant SARS-CoV-2 (COVID-19) NSP14 Methyltransferase (5925-6452) was expressed in E. coli using a C-terminal His-tag.
Catalog No. C19NS-E311H


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Catalog No. Pack Size Price (USD)
C19NS-E311H-10 10 ug $226
C19NS-E311H-20 20 ug $320
C19NS-E311H-50 50 ug $450
C19NS-E311H-BULK BULK Contact Us  


Overview:

Coronaviruses codes for a bifunctional non-structural protein 14 (NSP14) that is important for viral replication and transcription. NSP14 contains an N-terminal exo-ribonuclease (ExoN) domain for proof reading during viral replication and a C-terminal N-7 methyltransferase (N7-MTase) domain for mRNA capping. NSP14 associates with several viral proteins (1). It can bind to NSP10 and the resulting complex exhibits enhanced ExoN activity in vitro (2). It can also bind to the polymerase complex (NSP12/NSP8/NSP7) forming a complex that retains all associated enzymatic activities (3).


Gene Aliases:

NSP14: Non-structural protein 14


Genbank Number:


References:


1. Ma, Y. et al: Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex. PNAS, USA. (2015), 112(30):9436–9441.

2. Bouvet, M. et al: RNA 3′-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex. Proc Natl Acad Sci USA. (2012), 109:9372–9377.

3. Subissi, L. et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. Proc Natl Acad Sci USA. (2014), 111:E3900–E3909.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see the related technical datasheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical datasheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

60 kDa



Product Datasheets



There are no related publications available for this product.


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