Recombinant full-length 2019-nCoV Nucleocapsid (N) Protein was expressed in E. coli cells with a C-terminal His tag.
Catalog No. C19NP-G242H
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CoV N-protein is an RNA-binding protein primarily involved in binding to the viral RNA genome during packaging of viral RNA into a helical ribonucleoproteins or nucelocapsid structure (1). In addition, N-protein regulates viral RNA synthesis during replication/transcription and modulates host-pathogen interactions, such as such as actin reorganization, host cell cycle progression, and apoptosis (2). N-protein is abundantly expressed during infection and antibodies against N-protein have been detected in confirmed cases of Covid-19. Therefore, N-protein is a potent antigen that provides basis for future vaccine and diagnostic kit development (3).
Nucleoprotein, Nucleocapsid protein, NC, Protein N.
1. McBride R, et al: The coronavirus nucleocapsid is a multifunctional protein. Viruses. 2014, 6:2991-3018.
2. Surjit M, et al: The nucleocapsid protein of severe acute respiratory syndrome-coronavirus inhibits the activity of cyclin-cyclin-dependent kinase complex and blocks S phase progression in mammalian cells. J Biol Chem. 2006, 281:10669-10681
3. Zeng W, et al: Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochem Biophys Res Commun. 2020, 527(3):618‐623.
Binding of Anti-2019-nCoV N-Protein Antibody (C19NP-60H) to immobilized 2019-nCoV Nucleocapsid (N) Protein (C19NP-G242H) determined by ELISA using Goat anti-human Fc-HRP.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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