Specificity:

Recognizes proteins with N^G-mono-methylated arginine residues.

Cross Reactivity:

Pan-specific antibody. No reaction to acetylated proteins, di-methylated and tri-methylated proteins.

Host:

Rabbit

Immunogen:

N^G-mono-methylated arginine-KLH conjugates

Purification:

By affinity chromatography on a mono-methyl arginine agarose column

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot of the mono-methylated arginine protein profile with Anti-Methylated Arginine (N^G-Mono-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional mono-methyl arginine peptide inhibitor (B).

Sample Data:

Immunofluorecent stain of human melanoma (MMRU) cell with Anti-Methylated Arginine (N^G-Mono-methyl).