LOXL1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human LOXL1 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. L261-911
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LOXL1 is a member of the lysyl oxidase gene family which is essential to the biogenesis of connective tissue. LOXL1 encodes an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. LOXL1 is responsible for catalyzing the oxidative deamination of lysine residues of tropoelastin and this deamination causes spontaneous cross-linking and formation of elastin polymer fibers (1). LOXL1 serves both as a crosslinking enzyme and an element of the scaffold to ensure spatially defined deposition of elastin (2).
1. Hewitt, A. W.et.al: Ancestral LOXL1 variants are associated with pseudoexfoliation in Caucasian Australians but with markedly lower penetrance than in Nordic people. Hum. Molec. Genet. 17: 710-716, 2008.
2. Liu, X. et.al: Elastic fiber homeostasis requires lysyl oxidase-like 1 protein. Nature Genet. 36: 178-182, 2004.
LOXL1 siRNAs are designed to specifically knock-down human LOXL1 expression.
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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