Recombinant full-length human p110 alpha (N345K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Catalog No. P27-122CH
|Catalog No.||Pack Size||Price (USD)|
The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling (2).
p110 alpha: PIK3CA, MGC142161, MGC142163, p110-alpha
p85 alpha: PIK3R1, GRB1, p85-ALPHA
1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.
2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
p110 alpha(N345K) ~111 kDa and p85 alpha ~86 kDa
Y Zheng et al., Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Nature July 2013 10.1038/nature12308
Kong Kathleen et al., The human adenovirus E4-ORF1 protein subverts discs large 1 to mediate membrane recruitment and dysregulation of phosphatidylinositol 3-kinase PLoS Pathogens May 2014 10.1371/journal.ppat.1004102
Yalcin Abdullah et al., Nuclear Targeting of 6-Phosphofructo-2-kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases* Journal of Biological Chemistry November 2012 10.1074/jbc.M109.016816
M. Singel Stina et al., KIF14 Promotes AKT Phosphorylation and Contributes to Chemoresistance in Triple-Negative Breast Cancer Neoplasia March 2014 10.1016/j.neo.2014.03.008
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
Vidugiriene Jolanta et al., Evaluating the Utility of a Bioluminescent ADP-Detecting Assay for Lipid Kinases ASSAY and Drug Development Technologies January 2010 10.1089/adt.2009.0223
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling