1. Innis M A, et al: PCR protocols and applications: A laboratory manual, academic, New York, 1989. 2. Innis M A, et al: DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Proc. Natl. Acad. Sci. USA. 1988, 85:9436-9440. 3. Weyant R S, et al: Effect of ionic and nonionic detergents on the Taq polymerase. Biotechniques. 1990, 9:308-309.
Taq DNA polymerase is a highly thermostable DNA polymerase of Thermus aquaticus. Recombinant Taq DNA polymerase was expressed in E. Coli. The enzyme exhibits 5’→3’ polymerase activity and possesses 5’→3’ exonuclease activity, but has no detectable 3’→5’ exonuclease activity. In addition, Taq DNA polymerase generates PCR products containing extra adenines at the 3’-ends. The resulted products can be used for TA cloning.
Storage and Stability:
1.5yr at –20 oC from date of shipment
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