Recombinant tag-free Sau DNA polymerase large fragment from Staphylococcus aureus was expressed in E. coli cells.
Catalog No. SD01-E311U
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| SD01-E311U-200 | 200 U | $390 | |
| SD01-E311U-1000 | 1000 U | $525 | |
| SD01-E311U-BULK | BULK | Contact Us |
Overview:
DNA polymerase (DNAP) is an enzyme that catalyzes the DNA molecules and is crucial for DNA replication. Sau DNA polymerase large fragment is a product of the Staphylococcus aureus DNA polymerase I that retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ and 3´→ 5´ exonuclease activity (1). Sau DNA polymerase has a wide range of applications such as primer labelling, second-strand cDNA synthesis, DNA strand displacement synthesis, recombinase polymerase amplification, and single dA tailing (2).
Gene Aliases:
polA, DNA polymerase I
Genbank Number:
References:
1. Okazaki, T. et al. J. Biol.Chem. 1964, 239: 259-268. 2. Piepenburg, O. et al. PLOSBiology. 2006, 4: 1115-1121.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Activity:
5 U/µL
Unit definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
There are no related publications available for this product.
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