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Sau DNA Polymerase Large Fragment

Recombinant tag-free Sau DNA polymerase large fragment from Staphylococcus aureus was expressed in E. coli cells.
Catalog No. SD01-E311U


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Catalog No. Pack Size Price (USD)
SD01-E311U-200 200 U $390
SD01-E311U-1000 1000 U $525
SD01-E311U-BULK BULK Contact Us  


Overview:

DNA polymerase (DNAP) is an enzyme that catalyzes the DNA molecules and is crucial for DNA replication. Sau DNA polymerase large fragment is a product of the Staphylococcus aureus DNA polymerase I that retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ and 3´→ 5´ exonuclease activity (1). Sau DNA polymerase has a wide range of applications such as primer labelling, second-strand cDNA synthesis, DNA strand displacement synthesis, recombinase polymerase amplification, and single dA tailing (2).


Gene Aliases:

polA, DNA polymerase I


Genbank Number:


References:

1. Okazaki, T. et al. J. Biol.Chem. 1964, 239: 259-268. 2. Piepenburg, O. et al. PLOSBiology. 2006, 4: 1115-1121.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Activity:

5 U/µL


Unit definition:

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.


Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.



Product Datasheets



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