Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
Catalog No. P47-10H
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P47-10H-05 | 5 ug | $435 | |
| P47-10H-10 | 10 ug | $655 | |
| P47-10H-BULK | BULK | Contact Us |
Overview:
AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Gene Aliases:
Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666
References:
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.
2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cell Eur J Biochem. 1997 Jun 1;246(2):259-73.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~68kDa (A1), ~38kDa (B1), ~40kDa (G1)
Guha P et al., IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration
Cell Reports March 2019 10.1016/j.celrep.2019.02.013
Shuai Z et al., Cellular energy stress induces AMPK-mediated regulation of glioblastoma cell proliferation by PIKE-A phosphorylation
Cell death & disease March 2019 10.1038/s41419-019-1452-1
Liu X et al., AMPK-mediated degradation of Nav1. 5 through autophagy
The FASEB Journal April 2019 10.1096/fj.201801583RR
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
G Mahapatra et al., Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain Activity in Kidney: Implications for AMP Kinase. Journal of Biological Chemistry October 2016 10.1074/jbc.M116.744664
Meltzer-Mats Ella et al., Synthesis and Mechanism of Hypoglycemic Activity of Benzothiazole Derivatives Journal of Medicinal Chemistry June 2013 10.1021/jm4001488
Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009
Choi Jiwon et al., Sanguinarine is an allosteric activator of AMP-activated protein kinase Biochemical and Biophysical Research Communications September 2011 10.1016/j.bbrc.2011.08.081
L. Figarola James et al., Small?molecule COH-SR4 inhibits adipocyte differentiation via AMPK activation International Journal of Molecular Medicine March 2013 10.3892/ijmm.2013.1313
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
et al., Nucleus-translocated ACSS2 promotes the gene transcription for lysosomal biogenesis and autophagy Molecular cell June 2018
et al., 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation The Journal of Biological Chemistry February 2020
et al., The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62 Science Advances April 2019
et al., The PLAG1-GDH1 axis promotes anoikis resistance and tumor metastasis through CamKK2-AMPK signaling in LKB1-deficient lung cancer Molecular cell January 2019
et al., 2-[2-(4-(trifluoromethyl)phenylamino)thiazol-4-yl]acetic acid (Activator-3) is a potent activator of AMPK Scientific Reports June 2018
et al., A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells Scientific Reports February 2018
et al., AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes Redox Biology November 2019
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases
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