Recombinant Human Midkine (NEGF2) was expressed in E. coli cells.
Catalog No. M838-40N
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| M838-40N-20 | 20 ug | $215 | |
| M838-40N-100 | 100 ug | $665 | |
| M838-40N-1000 | 1 mg | $3400 | |
| M838-40N-BULK | BULK | Contact Us |
Overview:
Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.
Gene Aliases:
MK, NEGF-2
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight:
14.0 kDa
Reconstitution Protocol:
Reconstitute at 0.1 mg/ml in sterile PBS
Sample Cell Assay Data:
The ED (50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.
Purity:
Recombinant Human Midkine (NEGF2) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
|
There are no related publications available for this product.
Cancer
STAY CONNECTED
Fax: 1-604-232-4601
Toll Free: 1-866-954-6273
info@signalchem.com