Recombinant Human LIF was expressed in E. coli cells.
Catalog No. L845-40N
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L845-40N-10 | 10 ug | $215 | |
L845-40N-100 | 100 ug | $820 | |
L845-40N-1000 | 1 mg | $3163 | |
L845-40N-BULK | BULK | Contact Us |
Overview:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Gene Aliases:
LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Recombinant Human Leukemia Inhibitory Factor (LIF)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
20.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 was in found to be <0.05 ng as determined by the dose-dependent proliferation of human erythroleukemic cell line.
Purity:
Recombinant Human LIF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
There are no related publications available for this product.
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