Recombinant Human IL4 was expressed in E. coli cells.
Catalog No. I864-40N
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| I864-40N-10 | 10 ug | $215 | |
| I864-40N-100 | 100 ug | $995 | |
| I864-40N-1000 | 1 mg | $6950 | |
| I864-40N-BULK | BULK | Contact Us |
Overview:
IL4 is produced mainly by a sub-population of activated T helper cells that also secrete IL5 and IL6. The biological activities of IL4 are mediated by a specific receptor, the extracellular domain of which is related to the receptors for EPO, IL6, and the beta chain of the IL2 receptor. IL4 participates in several B-cell activation processes as well as of other cell types. A co-stimulator of DNA-synthesis, IL4 induces the expression of class II MHC molecules on resting B-cells, enhances both secretion and cell surface expression of IgE and IgG1, and also regulates the expression of the low affinity Fc receptor for IgE on both lymphocytes and monocytes. Pre-treatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma.
Gene Aliases:
IL-4, BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, Recombinant Human Interleukin-4 (IL4)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
15.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is ≤ 0.1 ng/Ml
Purity:
Recombinant Human IL4 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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