Recombinant Human IL2 was expressed in E. coli cells.
Catalog No. I862-40BN
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| I862-40BN-50 | 50 ug | $193 | |
| I862-40BN-100 | 100 ug | $358 | |
| I862-40BN-1000 | 1 mg | $2063 | |
| I862-40BN-BULK | BULK | Contact Us |
Overview:
A helical bundle of 4 α-helices, IL2 is an immunoregulatory cytokine that is expressed by CD4+/CD8+ T cells, γδ T cells, B cells, eosinophils and dendritic cells. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. While Human IL2 shares 56% and 66% amino acid sequence identity with mouse and rat IL2, respectively only human and mouse IL2 exhibit cross-species activity. Characteristically, IL2 exerts both autocrine and paracrine activity on T cells. In a regulatory role, IL2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Recombinant Human IL2 is a non-glycosylated polypeptide that contains one intrachain disulfide bond.
Gene Aliases:
IL-2, T-cell growth factor, TCGF
Recombinant Human Interleukin-2 (IL2)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
15.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the dose dependent proliferation of human T cells was found to be less than 0.5 ng/mL
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Purity:
Recombinant Human IL2 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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There are no related publications available for this product.
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