Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight:

17.0 kDa

Reconstitution Protocol:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Sample Cell Assay Data:

The ED50 was determined by the ability to inhibit the IL-1 alpha mediated cell proliferation in a murine helper T cell line, for this effect, in the presence of 50 pg/mL of IL-1 alpha, is typically 20-60 ng/mL

Purity:

Recombinant Human IL1RN resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250