Recombinant Human VEGF (165aa) was expressed in E. coli cells.
Catalog No. V815-40N
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| V815-40N-10 | 10 ug | $193 | |
| V815-40N-BULK | BULK | Contact Us |
Overview:
VEGF is a homodimeric glycoprotein that is actively involved in angiogenesis, vasculogenesis and endothelial cell growth. Owing to alternative splicing of the mRNA, VEGF occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 and 206 amino acids. The various isoforms of VEGF differ in biological properties such as; (a) the receptors they recognize and (b) their interaction with heparan sulfate proteoglycans. VEGF-121 and VEGF-165 are soluble secreted forms of the factor while VEGF-189 and VEGF-206 are mostly bound to heparin-containing proteoglycans in the cell surface or in the basement membrane. VEGF-165 is the most common variant in most tissues and one with heparin binding properties. Recombinant VEGF-165aa is a non-glycosylated, disulfide linked homodimer.
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
19.0 kDa
Gene Aliases :
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGF-165aa, Recombinant Human Vascular Endothelial Growth Factor 165AA (VEGFA)
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration no less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the dose-dependent proliferation of human umbilical vein endothelial cells, using a concentration range of 1-100 ng/mL, was found to be <10ng/mL
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Purity:
Recombinant Human VEGF (165aa) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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There are no related publications available for this product.
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