Recombinant Human TNF was expressed in E. coli cells.
Catalog No. T860-40BN
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| T860-40BN-50 | 50 ug | $193 | |
| T860-40BN-100 | 100 ug | $325 | |
| T860-40BN-1000 | 1 mg | $1601 | |
| T860-40BN-BULK | BULK | Contact Us |
Overview:
Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa.
Gene Aliases:
TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, Recombinant Human Tumor Necrosis Factor Alpha (TNF)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
17.6 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL
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Purity:
Recombinant Human TNF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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There are no related publications available for this product.
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