Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight:

20.1 kDa

Reconstitution Protocol:

A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Sample Cell Assay Data:

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1­ - 0.4 µg/mL.

Purity:

Recombinant Human SHH resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250