Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight:

20.0 kDa

Reconstitution Protocol:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Sample Cell Assay Data:

Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be less than 0.2ng/ml.

Purity:

Recombinant Human EPO resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.